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reference strain vr2332  (ATCC)


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    Structured Review

    ATCC reference strain vr2332
    Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or <t>VR2332</t> prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.
    Reference Strain Vr2332, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+atcc+vr2332/Lactobacillus+johnsonii/pmc12568354-79-2-5
    Average 95 stars, based on 293 article reviews
    reference strain vr2332 - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Mitochondrial dysfunction in PRRSV-2-infected macrophages"

    Article Title: Mitochondrial dysfunction in PRRSV-2-infected macrophages

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1670488

    Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or VR2332 prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.
    Figure Legend Snippet: Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or VR2332 prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.

    Techniques Used: Infection, Control, Derivative Assay, RNA Sequencing, Negative Control, Functional Assay, Virus

    Related Articles

    Immunofluorescence:

    Article Title: Porcine reproductive and respiratory syndrome virus: morphological, biochemical and serological characteristics of Quebec isolates associated with acute and chronic outbreaks of porcine reproductive and respiratory syndrome.
    Article Snippet: .. Serological identification of the virus was confirmed by indirect immunofluorescence and indirect protein A-gold immunoelectron microscopy using reference antiserum prepared from experimentally-infected specific pathogen free (SPF) piglets and monoclonal antibodies (MoAbs) directed against the p15 nucleocapsid (N) protein of the reference ATCC-VR2332 isolate. ..

    Article Title: Identification et caractérisation moléculaire des protéines structurales majeures du virus du syndrome reproducteur et respiratoire du porc (SRRP)
    Article Snippet: .. Serological identification of the virus was confirmed by indirect immunofluorescence and indirect protein A-gold immunoelectron microscopy using reference antiserum prepared from experimentally-infected specific pathogen free (SPF) piglets and monoclonal antibodies (MoAbs) directed against the p15 nucleocapsid (N) protein of the reference ATCC-VR2332 isolate. ..

    Immuno-Electron Microscopy:

    Article Title: Porcine reproductive and respiratory syndrome virus: morphological, biochemical and serological characteristics of Quebec isolates associated with acute and chronic outbreaks of porcine reproductive and respiratory syndrome.
    Article Snippet: .. Serological identification of the virus was confirmed by indirect immunofluorescence and indirect protein A-gold immunoelectron microscopy using reference antiserum prepared from experimentally-infected specific pathogen free (SPF) piglets and monoclonal antibodies (MoAbs) directed against the p15 nucleocapsid (N) protein of the reference ATCC-VR2332 isolate. ..

    Article Title: Identification et caractérisation moléculaire des protéines structurales majeures du virus du syndrome reproducteur et respiratoire du porc (SRRP)
    Article Snippet: .. Serological identification of the virus was confirmed by indirect immunofluorescence and indirect protein A-gold immunoelectron microscopy using reference antiserum prepared from experimentally-infected specific pathogen free (SPF) piglets and monoclonal antibodies (MoAbs) directed against the p15 nucleocapsid (N) protein of the reference ATCC-VR2332 isolate. ..

    Virus:

    Article Title: Identification et caractérisation moléculaire des protéines structurales majeures du virus du syndrome reproducteur et respiratoire du porc (SRRP)
    Article Snippet: .. Serological identification of the virus was confirmed by indirect immunofluorescence and indirect protein A-gold immunoelectron microscopy using reference antiserum prepared from experimentally-infected specific pathogen free (SPF) piglets and monoclonal antibodies (MoAbs) directed against the p15 nucleocapsid (N) protein of the reference ATCC-VR2332 isolate. ..

    Article Title: Identification et caractérisation moléculaire des protéines structurales majeures du virus du syndrome reproducteur et respiratoire du porc (SRRP)
    Article Snippet: However, a diffuse and granular cytoplasmic (mostly perinuclear) fluorescence was observed using convalescent sera from experimentally-infected SPF piglets that had been found previously to be positive for antibodies to the Lelystad and/or American ATCC-VR2332 isolates of PRRSV. .. Three monoclonal antibodies, SDOW17, EP147 and V017, directed against the nucleocapsid protein of the reference ATCC-VR2332 isolate, reacted positively with similar titers to different Quebec isolates of the virus. ..

    Article Title: Porcine reproductive and respiratory syndrome virus: morphological, biochemical and serological characteristics of Quebec isolates associated with acute and chronic outbreaks of porcine reproductive and respiratory syndrome.
    Article Snippet: .. Serological identification of the virus was confirmed by indirect immunofluorescence and indirect protein A-gold immunoelectron microscopy using reference antiserum prepared from experimentally-infected specific pathogen free (SPF) piglets and monoclonal antibodies (MoAbs) directed against the p15 nucleocapsid (N) protein of the reference ATCC-VR2332 isolate. ..

    Bioprocessing:

    Article Title: Identification et caractérisation moléculaire des protéines structurales majeures du virus du syndrome reproducteur et respiratoire du porc (SRRP)
    Article Snippet: .. Serological identification of the virus was confirmed by indirect immunofluorescence and indirect protein A-gold immunoelectron microscopy using reference antiserum prepared from experimentally-infected specific pathogen free (SPF) piglets and monoclonal antibodies (MoAbs) directed against the p15 nucleocapsid (N) protein of the reference ATCC-VR2332 isolate. ..

    Article Title: Identification et caractérisation moléculaire des protéines structurales majeures du virus du syndrome reproducteur et respiratoire du porc (SRRP)
    Article Snippet: However, a diffuse and granular cytoplasmic (mostly perinuclear) fluorescence was observed using convalescent sera from experimentally-infected SPF piglets that had been found previously to be positive for antibodies to the Lelystad and/or American ATCC-VR2332 isolates of PRRSV. .. Three monoclonal antibodies, SDOW17, EP147 and V017, directed against the nucleocapsid protein of the reference ATCC-VR2332 isolate, reacted positively with similar titers to different Quebec isolates of the virus. ..

    Article Title: Porcine reproductive and respiratory syndrome virus: morphological, biochemical and serological characteristics of Quebec isolates associated with acute and chronic outbreaks of porcine reproductive and respiratory syndrome.
    Article Snippet: .. Serological identification of the virus was confirmed by indirect immunofluorescence and indirect protein A-gold immunoelectron microscopy using reference antiserum prepared from experimentally-infected specific pathogen free (SPF) piglets and monoclonal antibodies (MoAbs) directed against the p15 nucleocapsid (N) protein of the reference ATCC-VR2332 isolate. ..

    Article Title: Identification et caractérisation moléculaire des protéines structurales majeures du virus du syndrome reproducteur et respiratoire du porc (SRRP)
    Article Snippet: 75 The reference hyperimmune sera used in the present study were obtained from experimentally—infected specific-pathogen-free (SPF) piglets, and reacted positively by indirect immunofluorescence (IIF titers of 12128 to 1:256) to the tissue culture- adapted American isolate ATCC-VR2332 of PRRS virus (Dea et al., 1992b). .. Monoclonal antibodies (MoAbs) SDOW17, EP147 and V017, directed against the nucleocapsid protein of the reference ATCC-VR2332 isolate of PRRSV (Benfield er al., 1992b; Collins et al., 1992), were kindly provided to us by Drs D.A. ..



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    Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or <t>VR2332</t> prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.
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    Image Search Results


    Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or VR2332 prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.

    Journal: Frontiers in Immunology

    Article Title: Mitochondrial dysfunction in PRRSV-2-infected macrophages

    doi: 10.3389/fimmu.2025.1670488

    Figure Lengend Snippet: Experimental layout. Lungs were collected from influenza- and PRRSV-negative 8–12-week-old pigs. Mononuclear phagocytes (MNPs) from bronchoalveolar lavage (BAL) and parenchyma (PAR) were enriched using OptiPrep gradient. MNPs were infected with North Carolina (NC) PRRSV-2 strains NC134 and NC174 (MOI = 1) and control media for 12 h. Infected and control porcine alveolar macrophages (PAMs), pulmonary intravascular macrophages (PIMs), monocyte-derived dendritic cells (moDCs), and classical DCs (cDCs) were sorted, and RNA-seq was performed on each cell subset. Additional PAM and PIM sorted cells were used for qPCR and NanoString validations. For other assays, enriched MNPs were plated to allow macrophage adhesion for 2 h. PAMs and PIMs were subsequently infected with different NC PRRSV-2 strains (NC134, NC174, and NC144) or VR2332 prototype strain; uninfected cells were used as negative control. Infected macrophages were used in different assays: mitochondrial function assay (Seahorse Agilent technology) and ROS and NO production assays. Created in BioRender. Crisci, E (2025). https://BioRender.com/65x4hji . PRRSV, porcine reproductive and respiratory syndrome virus; MOI, multiplicity of infection; ROS, reactive oxygen species; NO, nitric oxide.

    Article Snippet: Additionally, the reference strain VR2332 (ATCC strain BIAH-001, GenBank accession ID U87392.3 , L5A.1) was used as the PRRSV-2 prototype strain.

    Techniques: Infection, Control, Derivative Assay, RNA Sequencing, Negative Control, Functional Assay, Virus